In this lesson, students work from lower to higher levels of scientific inquiry while studying enzyme kinetics in a practical, student-centered, flipped-classroom process. In addition to reviewing the importance of enzymes to biological systems and examining the factors that influence their activity, this is a good lesson to practice the scientific method, from replication of pre-designed experiments to asking questions, designing investigations, collecting and analyzing data, sharing findings, and engaging in peer review. Designed for higher-level classes, this lesson can be modified, simplified, or shortened for regular and honors classes.
Grade Level(s): 9, 10, 11, 12
Computers for Students, Basic Calculators, Microsoft Office
3 Hour(s) 30 Minute(s)
Resource supports reading in content area:Yes
Keywords: Enzymes, Catalysis, Bioenergetics, Temperature, Acidity, Competitive Inhibition, Substrate Concentration, Enzyme Concentration
Instructional Component Type(s):
Student Center Activity
Lesson Plan Template: General Lesson Plan
Learning Objectives: What should students know and be able to do as a result of this lesson?
- Students will review the relationship between enzymatic structure and function by studying factors that affect enzymatic activity.
- Students will evaluate the biological importance of factors that impact enzymatic activity.
- Students will conduct investigations to examine factors that impact enzymatic activity.
- Students will collect data, analyze it, draw conclusions, and present them to classmates using MLA-style reports and research presentations. They will also engage in peer review of each other's work.
Prior Knowledge: What prior knowledge should students have for this lesson?
- Understanding of molecular structure of proteins and the interrelated nature of structure and function of biological molecules.
- Understanding of changes in Gibbs free energy and of how they relate to life's endergonic and exergonic reactions
- Understanding of basic thermodynamics laws and how they apply to living systems
- Understanding of basic chemical kinetics and how it is affected by factors such as temperature, reactant availability, and the presence of catalysts
- Understanding of the scientific method and how it applies to the writing of formal lab reports
- Familiarity with criteria for MLA-style lab report writing
- Familiarity with descriptive statistics and statistical analysis of significance (optional)
Guiding Questions: What are the guiding questions for this lesson?
- What is the role and importance of enzymes in organisms?
- What factors affect enzymatic activity?
- What is the importance of enzymes in organisms?
- How can the scientific method help us answer questions?
Teaching Phase: How will the teacher present the concept or skill to students?
As part of a flipped-classroom design, many parts of the teaching phase are completed through online resources and videos to maximize time spent in guided and independent practice.
- The instructor will provide students with videos and resources to review content prior to the investigation.
- The instructor will provide students with videos and resources on the construction of MLA-style lab reports.
- The instructor will model the process of grading using the lab report rubric.
- The instructor will review expectations and assign a due date for each part of the process.
- Prior to first day of investigation, students will complete pre-lab activities, which include accessing resources to review content and laboratory procedures.
- Students who fail to complete required preparatory steps at home will have to do so in class before starting the investigation.
- Students will prepare handwritten procedures/design, materials list, predictions, and prepared data collection tables prior to investigation.
- Students will answer pre-lab questions and read lab instructions prior to beginning the investigation.
- The teacher will review/model procedures for all parts and lead discussions of results as seen by averaged class data.
See the attached files for more information:
- Teacher Files:
- Instructor's Guide & Reasoning
- General Assignment Rubric
- Student Resources
- Student instructions
- Lab Report Explanation
- Lab Report Template
- Lab Report General Criteria
- Lab Report Rubric
- Lab Report Sample
- Preparing a Research Abstract
Guided Practice: What activities or exercises will the students complete with teacher guidance?
- During Prelab activities, students will use virtual activities and videos to practice for the investigation.
- During Part A, students will complete an experiment and analyze the results by following instructions written in a worksheet
- During Peer Review, students will follow the rubric and guides to evaluate the work of others.
Independent Practice: What activities or exercises will students complete to reinforce the concepts and skills developed in the lesson?
- Students will complete pre-lab questions, conceptual questions, and reflection questions at different points of the investigation
- Students will take a pre-lab quiz before conducting investigation
- Students will design and conduct their own experiments to explore topic
- Students will write and submit a formal MLA-style report
- Students will conduct peer-reviews of each other's work
Closure: How will the teacher assist students in organizing the knowledge gained in the lesson?
- Students will write lab reports and organize them together with other tasks performing during the investigation into a final document.
- Students will answer conclusion questions, write reflections, and participate in class discussions.
Submission of final document containing all of the following:
- Answers for several questions reviewing conceptual points/content associated with the topic explored in the investigation
- For Part A, completed laboratory worksheets
- For Part B, an organized, typed, formal MLA style report submitted hrough a plagiarism scanner and shared virtual portfolio. (Alternatively: Students will turn in bound paper submissions)
- For Part C, copies of peer-reviews completed
- Several reflection questions completed at the conclusion of the investigation
- MLA-style cover page, headers, citations, reference page, and work-cited page
- Prior to beginning the investigation, students will complete pre-lab questions. Their ability to answer these questions, as well as completion of preparation tasks (which include reading lab instructions), should serve as indicator of their readiness level.
- Prior to beginning the investigation, students will take a short pre-lab quiz based on required preparation tasks, lab procedures, and background information.
- Before each part, the instructor will verify that students are prepared for the laboratory day by having them complete handwritten procedures/design, materials list, predictions, and prepared data collection tables.
- Instructor will monitor students during experimentation and guide them to make sure all students are focused and complete the experiment carefully and accurately.
- Before Part B, students will submit research designs for instructor approval.
- After Part B, students will receive points for preliminary lab report submissions.
Feedback to Students
Prior to the investigation, the instructor will review pre-lab questions answers by leading a class discussion.
- Prior to the investigation, the instructor will check if students completed preparation tasks at home and guide students in their process of doing it if they failed to do them as required.
- The instructor will review pre-lab quiz questions with the class while students check each others' answers. Students who failed to pass may be required to retake quiz after completing or reviewing preparation tasks before they are allowed to continue to the investigation.
- The instructor will give feedback on student performance during experimentation and guide students to improve their attention, methods, and focus.
- The instructor will monitor group work activities to ensure all students are engaged and contributing to discussions and experimentation.
- Students will receive feedback on their conduct during experimentation.
- For Part A, the instructor will guide class discussion on reporting and analyzes of averaged class data.
- For Part B, students will receive feedback and guidance on their research design submission.
- After lab reports are submitted, students will receive feedback from peer-reviews and given the opportunity to correct their initial submission before the final document is due.
- Students will receive final feedback after submitting the final document.
Accommodations & Recommendations
Source and Access Information
Name of Author/Source: Jefferson Lima
District/Organization of Contributor(s): Miami-Dade
Access Privileges: Public
* Please note that examples of resources are not intended as complete curriculum.