SC.912.L.16.12

Describe how basic DNA technology (restriction digestion by endonucleases, gel electrophoresis, polymerase chain reaction, ligation, and transformation) is used to construct recombinant DNA molecules (DNA cloning).
General Information
Subject Area: Science
Grade: 912
Body of Knowledge: Life Science
Idea: Level 2: Basic Application of Skills & Concepts
Standard: Heredity and Reproduction -

A. DNA stores and transmits genetic information. Genes are sets of instructions encoded in the structure of DNA.

B. Genetic information is passed from generation to generation by DNA in all organisms and accounts for similarities in related individuals.

C. Manipulation of DNA in organisms has led to commercial production of biological molecules on a large scale and genetically modified organisms.

D. Reproduction is characteristic of living things and is essential for the survival of species.

Date Adopted or Revised: 02/08
Date of Last Rating: 05/08
Status: State Board Approved

Related Courses

This benchmark is part of these courses.
2000320: Biology 1 Honors (Specifically in versions: 2014 - 2015, 2015 - 2022, 2022 and beyond (current))
2000330: Biology 2 Honors (Specifically in versions: 2014 - 2015, 2015 - 2018, 2018 - 2022, 2022 and beyond (current))
2000430: Biology Technology (Specifically in versions: 2014 - 2015, 2015 - 2022, 2022 and beyond (current))
3027010: Biotechnology 1 (Specifically in versions: 2015 - 2022, 2022 and beyond (current))
3027020: Biotechnology 2 (Specifically in versions: 2015 and beyond (current))
2002480: Forensic Science 1 (Specifically in versions: 2014 - 2015, 2015 - 2017, 2017 - 2022, 2022 and beyond (current))
2002490: Forensic Sciences 2 (Specifically in versions: 2014 - 2015, 2015 - 2017, 2017 - 2022, 2022 and beyond (current))
2000440: Genetics Honors (Specifically in versions: 2014 - 2015, 2015 - 2022, 2022 and beyond (current))
2002430: Integrated Science 2 Honors (Specifically in versions: 2014 - 2015, 2015 - 2022, 2022 and beyond (current))
2000500: Bioscience 1 Honors (Specifically in versions: 2014 - 2015, 2015 - 2022, 2022 - 2023 (current), 2023 and beyond)
2000510: Bioscience 2 Honors (Specifically in versions: 2014 - 2015, 2015 - 2022, 2022 - 2023 (current), 2023 and beyond)

Related Access Points

Alternate version of this benchmark for students with significant cognitive disabilities.

Related Resources

Vetted resources educators can use to teach the concepts and skills in this benchmark.

Lesson Plans

Using DNA to Identify People:

 Learning objectives:  Students will learn what DNA fingerprinting is, what it is used for, and how it is used in paternity testing and forensics.  Students will see how this technique actually works in lab.  Students will learn how to analyze the gels used in this technique to match babies to parents, and crime scene evidence to suspects.

Type: Lesson Plan

How to be a Molecular Biologist the Easy Way:

This lesson plan details the ethical concepts of biotechnology and allows students to explore basic concepts of manipulating and analyzing DNA in a classroom setting. The lesson takes the students through a discussion of controversial topics related to molecular biology and biotechnology, DNA isolation, restriction digestion of DNA, gel electrophoresis, and DNA cloning.

Type: Lesson Plan

Perspectives Video: Experts

Rapid Genetic Identification of Sharks:

Dr. Mahmood Shivji explains how rapid genetic testing of shark tissue samples is used to address societal pressures on marine environments.

Download the CPALMS Perspectives video student note taking guide.

Type: Perspectives Video: Expert

DNA Microsatellite Analysis for Plant Ecology:

Dr. David McNutt explains how large clonal plant populations can be analyzed with microsatellite analysis of their DNA.

Download the CPALMS Perspectives video student note taking guide.

Type: Perspectives Video: Expert

Protein Factories:

What's in a molecular biologist's toolbox? Very small tools for working with cellular machines and molecules!

Download the CPALMS Perspectives video student note taking guide.

Type: Perspectives Video: Expert

Text Resources

Animal Clones: Double Trouble?:

This informational text resource is intended to support reading in the content area. Would you want to eat "clone chops?" This article discusses the possibility of food products derived from cloned animals appearing on our plates in the future. Also included is a brief history of cloning and the methods by which it is executed. In addition, the ethical and health arguments surrounding this development are discussed.

Type: Text Resource

"Designer" Chromosome for Brewer's Yeast Built from Scratch:

This informational text resource is intended to support reading in the content area. Scientists have been able to create a synthetic functioning chromosome (Saccharomyces cerevisiae) found in yeast. With this breakthrough, they might be able to create customizable bio-fuels, vaccines, or even synthetic organisms in the future.

Type: Text Resource

Tutorials

Polymerase Chain Reaction:

This tutorial will help you to understand the procedure of amplifying a single copy of DNA into millions of copies. Polymerase chain reaction is a molecular prototyping technique which helps in copying small segments of DNA into significant amounts required for molecular and genetic analyses.

Type: Tutorial

DNA Fingerprinting:

This tutorial will help you to visualize how DNA fingerprinting uses the pattern of DNA fragments caused by specific enzymes to identify individual organisms including humans, animals, plants or any other organism with DNA.

Type: Tutorial

Polymerase Chain Reaction:

This tutorial introduces the polymerase chain reaction (PCR), which is a technique used in molecular biology to make multiple copies of a gene even when only small amounts of DNA are available.

Type: Tutorial

Video/Audio/Animation

Lab: DNA Fingerprint: Alu:

  • Background on tracking human ancestry using the alu marker
  • Animation on polymerase chain reaction, PCR
  • Interactive activity for performing PCR

Type: Video/Audio/Animation

Student Resources

Vetted resources students can use to learn the concepts and skills in this benchmark.

Lesson Plan

Using DNA to Identify People:

 Learning objectives:  Students will learn what DNA fingerprinting is, what it is used for, and how it is used in paternity testing and forensics.  Students will see how this technique actually works in lab.  Students will learn how to analyze the gels used in this technique to match babies to parents, and crime scene evidence to suspects.

Type: Lesson Plan

Perspectives Video: Expert

Protein Factories:

What's in a molecular biologist's toolbox? Very small tools for working with cellular machines and molecules!

Download the CPALMS Perspectives video student note taking guide.

Type: Perspectives Video: Expert

Tutorials

Polymerase Chain Reaction:

This tutorial will help you to understand the procedure of amplifying a single copy of DNA into millions of copies. Polymerase chain reaction is a molecular prototyping technique which helps in copying small segments of DNA into significant amounts required for molecular and genetic analyses.

Type: Tutorial

DNA Fingerprinting:

This tutorial will help you to visualize how DNA fingerprinting uses the pattern of DNA fragments caused by specific enzymes to identify individual organisms including humans, animals, plants or any other organism with DNA.

Type: Tutorial

Polymerase Chain Reaction:

This tutorial introduces the polymerase chain reaction (PCR), which is a technique used in molecular biology to make multiple copies of a gene even when only small amounts of DNA are available.

Type: Tutorial

Video/Audio/Animation

Lab: DNA Fingerprint: Alu:

  • Background on tracking human ancestry using the alu marker
  • Animation on polymerase chain reaction, PCR
  • Interactive activity for performing PCR

Type: Video/Audio/Animation

Parent Resources

Vetted resources caregivers can use to help students learn the concepts and skills in this benchmark.

Perspectives Video: Expert

Protein Factories:

What's in a molecular biologist's toolbox? Very small tools for working with cellular machines and molecules!

Download the CPALMS Perspectives video student note taking guide.

Type: Perspectives Video: Expert