Standard #: SC.912.L.16.12


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Describe how basic DNA technology (restriction digestion by endonucleases, gel electrophoresis, polymerase chain reaction, ligation, and transformation) is used to construct recombinant DNA molecules (DNA cloning).


General Information

Subject Area: Science
Grade: 912
Body of Knowledge: Life Science
Standard: Heredity and Reproduction -

A. DNA stores and transmits genetic information. Genes are sets of instructions encoded in the structure of DNA.

B. Genetic information is passed from generation to generation by DNA in all organisms and accounts for similarities in related individuals.

C. Manipulation of DNA in organisms has led to commercial production of biological molecules on a large scale and genetically modified organisms.

D. Reproduction is characteristic of living things and is essential for the survival of species.

Date Adopted or Revised: 02/08
Content Complexity Rating: Level 2: Basic Application of Skills & Concepts - More Information
Date of Last Rating: 05/08
Status: State Board Approved

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Related Resources

Lesson Plans

Name Description
Who Regulates It?: The Government’s Role in Biotechnology

Students will learn about some applications of various biotechnology advancements by reading a series of scenarios. They will evaluate some ethical, legal, and moral implications of biotechnology, including if or how it can be regulated by the government in this integrated lesson plan.

Using DNA to Identify People

 Learning objectives:  Students will learn what DNA fingerprinting is, what it is used for, and how it is used in paternity testing and forensics.  Students will see how this technique actually works in lab.  Students will learn how to analyze the gels used in this technique to match babies to parents, and crime scene evidence to suspects.

How to be a Molecular Biologist the Easy Way

This lesson plan details the ethical concepts of biotechnology and allows students to explore basic concepts of manipulating and analyzing DNA in a classroom setting. The lesson takes the students through a discussion of controversial topics related to molecular biology and biotechnology, DNA isolation, restriction digestion of DNA, gel electrophoresis, and DNA cloning.

Perspectives Video: Experts

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Rapid Genetic Identification of Sharks

Dr. Mahmood Shivji explains how rapid genetic testing of shark tissue samples is used to address societal pressures on marine environments.

Download the CPALMS Perspectives video student note taking guide.

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Download the CPALMS Perspectives video student note taking guide.

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Download the CPALMS Perspectives video student note taking guide.

Text Resources

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Animal Clones: Double Trouble?

This informational text resource is intended to support reading in the content area. Would you want to eat "clone chops?" This article discusses the possibility of food products derived from cloned animals appearing on our plates in the future. Also included is a brief history of cloning and the methods by which it is executed. In addition, the ethical and health arguments surrounding this development are discussed.

"Designer" Chromosome for Brewer's Yeast Built from Scratch

This informational text resource is intended to support reading in the content area. Scientists have been able to create a synthetic functioning chromosome (Saccharomyces cerevisiae) found in yeast. With this breakthrough, they might be able to create customizable bio-fuels, vaccines, or even synthetic organisms in the future.

Tutorials

Name Description
Polymerase Chain Reaction

This tutorial will help you to understand the procedure of amplifying a single copy of DNA into millions of copies. Polymerase chain reaction is a molecular prototyping technique which helps in copying small segments of DNA into significant amounts required for molecular and genetic analyses.

Polymerase Chain Reaction

This tutorial introduces the polymerase chain reaction (PCR), which is a technique used in molecular biology to make multiple copies of a gene even when only small amounts of DNA are available.

Video/Audio/Animation

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Lab: DNA Fingerprint: Alu
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Student Resources

Lesson Plan

Name Description
Using DNA to Identify People:

 Learning objectives:  Students will learn what DNA fingerprinting is, what it is used for, and how it is used in paternity testing and forensics.  Students will see how this technique actually works in lab.  Students will learn how to analyze the gels used in this technique to match babies to parents, and crime scene evidence to suspects.

Perspectives Video: Expert

Name Description
Protein Factories:

What's in a molecular biologist's toolbox? Very small tools for working with cellular machines and molecules!

Download the CPALMS Perspectives video student note taking guide.

Tutorials

Name Description
Polymerase Chain Reaction:

This tutorial will help you to understand the procedure of amplifying a single copy of DNA into millions of copies. Polymerase chain reaction is a molecular prototyping technique which helps in copying small segments of DNA into significant amounts required for molecular and genetic analyses.

Polymerase Chain Reaction:

This tutorial introduces the polymerase chain reaction (PCR), which is a technique used in molecular biology to make multiple copies of a gene even when only small amounts of DNA are available.

Video/Audio/Animation

Name Description
Lab: DNA Fingerprint: Alu:
  • Background on tracking human ancestry using the alu marker
  • Animation on polymerase chain reaction, PCR
  • Interactive activity for performing PCR


Parent Resources

Perspectives Video: Expert

Name Description
Protein Factories:

What's in a molecular biologist's toolbox? Very small tools for working with cellular machines and molecules!

Download the CPALMS Perspectives video student note taking guide.



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